구강암 세포주를 이종이식한 설암의 동소위 누드마우스 모델
An orthotopic nude mouse model of tongue carcinoma
정재승, 김소미, 차인호, 황영선, 장향란,
소속 상세정보
정재승 ( Chung Jae-Seung ) - 연세대학교 치과대학 구강악안면외과학교실
김소미 ( Kim So-Mi ) - 연세대학교 치과대학 구강악안면외과학교실
차인호 ( Cha In-Ho ) - 연세대학교 치과대학 구강악안면외과학교실
황영선 ( Hwang Young-Sun ) - 연세대학교 치과대학 구강종양연구소
장향란 ( Zhang Xiang Lan ) - 연세대학교 치과대학 구강종양연구소
KMID : 0355620110370060490
Abstract
Introduction: Development of carcinoma on oral tongue may cause bilateral cervical lymph node metastasis, rapid invasion and growth of the cancer cells due to rich blood supply in muscle tissues. It is not only difficult to develop an animal experimental model, but also to proceed follow-up research after the development of such model as the induction of cancer lead to difficulty in taking nutrition for the experimental animals that often causes early death.
Materials and Methods : IIn this study, author have transplanted YD-10Bmod cells into nude mouse oral tongues with different cells number (5×104, 5×105, 5×106 cells/mouse) and observed the development aspect of oral tongue cancers.
Results: The cancer developed from orthotopic transplantation of YD-10Bmod cells into nude mouse oral tongue show invasion and central necrosis of the tumor, similar to the cancers developed human oral tongue cancer. The difference in tumor size and the time of central necrosis development depending on the number of transplanted tumor cells shows the feasibility of extending the survival period of the nude mouse by limiting the transplanted tumor cells to <5×104 cells/mouse or under per nude mouse.
Conclusion: This nude mouse model could be used effectively in developing effective chemotheray agent and establishing an animal experimental model that can be used to study the mechanism of cervical lymph node metastasis of the oral tongue cancer.
키워드
Tongue;Squamous cell carcinoma;Cell line
원문 및 링크아웃 정보
등재저널 정보